Take the Red Police to Marvel
Chapter 28 Cloning
The main purpose of coming to the Soviet Union this time is not only to sell technology but also to chat with her idol. Now that these two things have been completed, she has nothing to look forward to doing.
Now that Alvin has returned to the United States, he can do it, and Rhine doesn't have to run thousands of kilometers by himself.
Then after returning, she can use the production capacity vacated by the base vehicle to produce cutting-edge technology equipment. Now that it has been nearly a few years since the research and development of the super soldier drug, it is time to prepare for strengthening herself.
Because every time he is in the rear or commanding or researching, Rhine does not intend to strengthen his physical strength like the US team, but focuses on life span.
Theoretically, conventional super-soldier potions are expected to prolong the lifespan of humans by about 100 years. If the body enhancement content inside is genetically redirected, then this number can be extended to 150 years.
Of course, this is only theoretical, and everything needs more specific experiments to verify.Rheinland has been researching theoretical things over the years, and now all he needs is equipment to carry out experiments.
The experiment this time is the same as before, all human experiments are required, but Rhine will not use ordinary people who are unfamiliar, and the subject of the experiment is herself.
Not technically herself, but her clone.
The physical condition of the clone is exactly the same as hers, and the strengthening medicine prepared for her will be completely suitable for her.
The Red Alert system also has technical reserves related to human cloning. There used to be a high-tech building called the Replication Center in the Soviet Union. Later, after Yuri defected, it was changed to the Yuri camp. Of course, this technology exists in the system.
The technical level required for cloning is far more difficult than Rhine imagined, especially the equipment for a series of precise operations, even the base vehicle can barely be produced.
In order to assemble a computing center capable of analyzing data, Rhein even bit the bullet and summoned several advanced technology units, and then disassembled and modified the chips inside.
It took nearly a year of tossing like this before she barely built the laboratory and prepared the necessary things.
After that, you need to prepare your own clone. The specific operation is a bit complicated, but the most basic thing is to remove the nucleus of the egg cell first.
There is one thing to say, the prism inside the Prism tank is really high quality, she disassembled it and modified it to handle this process.
First, an electron tunneling microscope is used to observe the egg cell, and the temporary data processing center is used to model and image the egg cell.
After locating the position of the cell nucleus, a laser can be used to drill a hole in the zona pellucida, and then a light cone can be used to remove the cell nucleus. After removing the cell nucleus, a micromanipulator can be used to insert her own somatic cell nucleus.
It's just because these devices were all temporarily made by herself and other engineers, and the accuracy and operability can only be said to be barely usable, so she successfully completed the implantation and electrochemical induction after many failures.
The overall difficulty of the first step is not particularly high, and after 12 days these embryos will grow to the blastocyst stage.
The next step is to transfer these blastocysts to the inside of the artificial womb and develop them. This sounds simple, but it is actually extremely difficult to do.
The rare thing is not the process of transplantation, but the process of development.
The process of human embryo development inside the womb is very complicated, uncontrollable and uncertain. To fully simulate this process, a computer must use powerful computing power to analyze the embryo in real time. The temporary computer she modified can barely meet this requirement. Require.
But another difficulty will not disappear because of reluctance, she needs to use to create an artificial endometrium, the material of this endometrium can be biodegradable 'polylactic acid glycolic acid polymer'.
There is no technical difficulty in the operation of this single thing, but because of equipment problems, it takes a very long time to produce a qualified artificial endometrium with probability.
The real rare thing is how to simulate the internal environment of the uterus and generate blood vessels that transmit nutrients like real human tissue. Of course, Laing took this into consideration. She manually induced the formation of a self-sufficient inner wall in the endometrium through artificial transplantation. Circulatory network, which is why the success rate of artificial endometrium production is so low.
After the embryo is placed, she only needs to connect it to a pre-assembled dialysis machine to inject fresh blood, separate out the old blood and add enough nutrients to the blood to realize the simulated intrauterine circulation.
This blood is definitely not real blood, but a perfluorotributylamine emulsion with the ability to carry oxygen.
This substance is completely artificially synthesized, and its oxygen solubility is even more than twice that of blood. It has a strong ability to dissolve oxygen. Later generations of medicine often use this substance as a substitute for blood.
The artificial endometrium must not be exposed outside, so it was placed in a culture chamber for the growth of clones. The constant temperature culture chamber was connected with two tubes to simulate the effect of venous arteries.
Put the artificial endometrium in and connect it well, and add the nutrient solution to prevent the endometrium from shriveling and withering, and the beginning of the cultivation is complete.
That's right, the beginning of cultivation, such complicated steps are just the beginning.
She must artificially extract minerals, glucose, amylase, urea, uric acid and other substances to synthesize amniotic fluid before the nutrients in the yolk are consumed in the early stages of embryonic rearing.Otherwise, the embryo will die due to lack of nutrition.
This process takes about [-] weeks. After [-] weeks, the vascular network of the artificial endometrium cannot meet the growth of the fetus, but at that time the fetus has formed the umbilical cord and placenta.
After having the umbilical cord, there is no need for the endometrium. She can directly infuse the blood vessels connected to the artificial lung oxygen synthesizer, and also send in the protein, vitamins, inorganic salts and other substances needed for growth. .
Looking at it this way, somatic cell implantation is actually the easiest part, because the control of nutrient input is really almost impossible to complete by manpower.
For this reason, Rhine wrote two sets of simple control algorithms to simulate the intrauterine environment and deliver nutrients to the fetus. However, due to time constraints, the algorithms were too simple, so she had to stay by the side and stare at the data all the time to guard against abnormal phenomena.
Rhine had to manually adjust the parameters for any movement of the fetus to adjust the nutrient supply to be above or below the average line, which was a very torturous task.
And none of those red police units really understand biological engineering. These engineers are good at building power stations, bridges, and urban planning, but it would be a bit embarrassing for them to do this.
Therefore, Rhine could only stay in the laboratory by himself while sorting out the experimental data and monitoring the situation to adjust the parameters. At first, the experiment almost failed because of the problem of the control system being too simple.
This situation did not improve a lot until the algorithm was continuously improved a week later. From 24 hours to 12 hours, it is already a very big improvement.
Rhine, who was staring at the data, had dark circles under her eyes, and she looked a lot thinner. She even drank cognitive enhancement potions for this little guy.
For a whole week, she only slept for about eight hours. To be honest, those pregnant women had a much easier time giving birth than herself.
Now that Alvin has returned to the United States, he can do it, and Rhine doesn't have to run thousands of kilometers by himself.
Then after returning, she can use the production capacity vacated by the base vehicle to produce cutting-edge technology equipment. Now that it has been nearly a few years since the research and development of the super soldier drug, it is time to prepare for strengthening herself.
Because every time he is in the rear or commanding or researching, Rhine does not intend to strengthen his physical strength like the US team, but focuses on life span.
Theoretically, conventional super-soldier potions are expected to prolong the lifespan of humans by about 100 years. If the body enhancement content inside is genetically redirected, then this number can be extended to 150 years.
Of course, this is only theoretical, and everything needs more specific experiments to verify.Rheinland has been researching theoretical things over the years, and now all he needs is equipment to carry out experiments.
The experiment this time is the same as before, all human experiments are required, but Rhine will not use ordinary people who are unfamiliar, and the subject of the experiment is herself.
Not technically herself, but her clone.
The physical condition of the clone is exactly the same as hers, and the strengthening medicine prepared for her will be completely suitable for her.
The Red Alert system also has technical reserves related to human cloning. There used to be a high-tech building called the Replication Center in the Soviet Union. Later, after Yuri defected, it was changed to the Yuri camp. Of course, this technology exists in the system.
The technical level required for cloning is far more difficult than Rhine imagined, especially the equipment for a series of precise operations, even the base vehicle can barely be produced.
In order to assemble a computing center capable of analyzing data, Rhein even bit the bullet and summoned several advanced technology units, and then disassembled and modified the chips inside.
It took nearly a year of tossing like this before she barely built the laboratory and prepared the necessary things.
After that, you need to prepare your own clone. The specific operation is a bit complicated, but the most basic thing is to remove the nucleus of the egg cell first.
There is one thing to say, the prism inside the Prism tank is really high quality, she disassembled it and modified it to handle this process.
First, an electron tunneling microscope is used to observe the egg cell, and the temporary data processing center is used to model and image the egg cell.
After locating the position of the cell nucleus, a laser can be used to drill a hole in the zona pellucida, and then a light cone can be used to remove the cell nucleus. After removing the cell nucleus, a micromanipulator can be used to insert her own somatic cell nucleus.
It's just because these devices were all temporarily made by herself and other engineers, and the accuracy and operability can only be said to be barely usable, so she successfully completed the implantation and electrochemical induction after many failures.
The overall difficulty of the first step is not particularly high, and after 12 days these embryos will grow to the blastocyst stage.
The next step is to transfer these blastocysts to the inside of the artificial womb and develop them. This sounds simple, but it is actually extremely difficult to do.
The rare thing is not the process of transplantation, but the process of development.
The process of human embryo development inside the womb is very complicated, uncontrollable and uncertain. To fully simulate this process, a computer must use powerful computing power to analyze the embryo in real time. The temporary computer she modified can barely meet this requirement. Require.
But another difficulty will not disappear because of reluctance, she needs to use to create an artificial endometrium, the material of this endometrium can be biodegradable 'polylactic acid glycolic acid polymer'.
There is no technical difficulty in the operation of this single thing, but because of equipment problems, it takes a very long time to produce a qualified artificial endometrium with probability.
The real rare thing is how to simulate the internal environment of the uterus and generate blood vessels that transmit nutrients like real human tissue. Of course, Laing took this into consideration. She manually induced the formation of a self-sufficient inner wall in the endometrium through artificial transplantation. Circulatory network, which is why the success rate of artificial endometrium production is so low.
After the embryo is placed, she only needs to connect it to a pre-assembled dialysis machine to inject fresh blood, separate out the old blood and add enough nutrients to the blood to realize the simulated intrauterine circulation.
This blood is definitely not real blood, but a perfluorotributylamine emulsion with the ability to carry oxygen.
This substance is completely artificially synthesized, and its oxygen solubility is even more than twice that of blood. It has a strong ability to dissolve oxygen. Later generations of medicine often use this substance as a substitute for blood.
The artificial endometrium must not be exposed outside, so it was placed in a culture chamber for the growth of clones. The constant temperature culture chamber was connected with two tubes to simulate the effect of venous arteries.
Put the artificial endometrium in and connect it well, and add the nutrient solution to prevent the endometrium from shriveling and withering, and the beginning of the cultivation is complete.
That's right, the beginning of cultivation, such complicated steps are just the beginning.
She must artificially extract minerals, glucose, amylase, urea, uric acid and other substances to synthesize amniotic fluid before the nutrients in the yolk are consumed in the early stages of embryonic rearing.Otherwise, the embryo will die due to lack of nutrition.
This process takes about [-] weeks. After [-] weeks, the vascular network of the artificial endometrium cannot meet the growth of the fetus, but at that time the fetus has formed the umbilical cord and placenta.
After having the umbilical cord, there is no need for the endometrium. She can directly infuse the blood vessels connected to the artificial lung oxygen synthesizer, and also send in the protein, vitamins, inorganic salts and other substances needed for growth. .
Looking at it this way, somatic cell implantation is actually the easiest part, because the control of nutrient input is really almost impossible to complete by manpower.
For this reason, Rhine wrote two sets of simple control algorithms to simulate the intrauterine environment and deliver nutrients to the fetus. However, due to time constraints, the algorithms were too simple, so she had to stay by the side and stare at the data all the time to guard against abnormal phenomena.
Rhine had to manually adjust the parameters for any movement of the fetus to adjust the nutrient supply to be above or below the average line, which was a very torturous task.
And none of those red police units really understand biological engineering. These engineers are good at building power stations, bridges, and urban planning, but it would be a bit embarrassing for them to do this.
Therefore, Rhine could only stay in the laboratory by himself while sorting out the experimental data and monitoring the situation to adjust the parameters. At first, the experiment almost failed because of the problem of the control system being too simple.
This situation did not improve a lot until the algorithm was continuously improved a week later. From 24 hours to 12 hours, it is already a very big improvement.
Rhine, who was staring at the data, had dark circles under her eyes, and she looked a lot thinner. She even drank cognitive enhancement potions for this little guy.
For a whole week, she only slept for about eight hours. To be honest, those pregnant women had a much easier time giving birth than herself.
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